Part:BBa_J61037:Design

pBAC872s

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4498
  Illegal XbaI site found at 6650
  Illegal PstI site found at 2050
  Illegal PstI site found at 5957
  Illegal PstI site found at 7479
  Illegal PstI site found at 9020
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4498
  Illegal NheI site found at 2209
  Illegal PstI site found at 2050
  Illegal PstI site found at 5957
  Illegal PstI site found at 7479
  Illegal PstI site found at 9020
  Illegal NotI site found at 6
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4498
  Illegal BglII site found at 6170
  Illegal BglII site found at 6604
  Illegal BglII site found at 8671
  Illegal BamHI site found at 1296
  Illegal XhoI site found at 70
  Illegal XhoI site found at 6471
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4498
  Illegal XbaI site found at 6650
  Illegal PstI site found at 2050
  Illegal PstI site found at 5957
  Illegal PstI site found at 7479
  Illegal PstI site found at 9020
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4498
  Illegal XbaI site found at 6650
  Illegal PstI site found at 2050
  Illegal PstI site found at 5957
  Illegal PstI site found at 7479
  Illegal PstI site found at 9020
  Illegal NgoMIV site found at 5506
  Illegal NgoMIV site found at 7161
  Illegal AgeI site found at 1080
  Illegal AgeI site found at 7285
  Illegal AgeI site found at 8515
  Illegal AgeI site found at 9408
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4591
  Illegal BsaI site found at 4769
  Illegal BsaI site found at 5335
  Illegal BsaI site found at 10262
  Illegal BsaI.rc site found at 1975
  Illegal BsaI.rc site found at 3641
  Illegal SapI site found at 1062
  Illegal SapI site found at 2558
  Illegal SapI site found at 5446
  Illegal SapI site found at 5656
  Illegal SapI site found at 8054
  Illegal SapI site found at 9264
  

Design Notes

N/A

Source

N/A